지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.11 No.2
- 발행연도
- 2006.6
- 수록면
- 127 - 132 (6page)
이용수
초록· 키워드
오류제보하기
Bacillus sp. strain K-1, which produces a strong fibrinolytic enzyme, was isolated from chongkukjang, a traditional Korean fermented soybean paste. The fibrinolytic enzyme was purified from chongkukjang base by using ammonium sulfate fractionation and chromatographic techniques. Purified enzyme, CK K-1 was demonstrated to be homogeneous by SDS-PAGE and isoelectric focusing electrophoresis, and has molecular mass of a 12.4 kDa and a pI of 8.0. The optimal reaction pH value and temperature were 8.0 and 40℃, respectively. Phenyl-methyl-sulfonyl-fluoride (PMSF; serine protease inhibitor), ethylene-diamine-tetra-acetic acid (EDTA; metallo protease inhibitor), copper ion, ferric ion and lead ion inhibited the enzyme activity. These results indicated that the fibrinolytic enzyme is a metallo-serine protease and different from nattokinase and chongkukjangkinase.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
참고문헌 (19)
참고문헌 신청
Voet D, Voet JG. 1990. Biochemistry. John Wiley & Sons, New York. p 1087-1095.
Wun TC, Schleuning WD, Reich E. 1982. Isolation and characterization of urokinase from human plasma. J Biol Chem 257: 3276-3283.
Medved LV, Solovjov DA, Ingham KC. 1996. Domain structure, stability and interaction in streptokinase. Eur J Biochem 239: 333-339.
Pennica D, Holmes WE, Kohr WJ, Harkins RN, Vehar GA, Ward CA, Bennn WF, Yelverton E, Seeburg PH, Heyneker HL, Goeddel DV, Cohen D. 1982. Clonning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 301: 214-221.
Sumi H, Hamada H, Tsushima H, Mihara H, Muraki H. 1987. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese natto: a typical and popular soybean food in the Japanese diet. Experientia 43: 1110-1111.
Wun TC, Schleuning WD, Reich E. 1982. Isolation and characterization of urokinase from human plasma. J Biol Chem 257: 3276-3283.
Medved LV, Solovjov DA, Ingham KC. 1996. Domain structure, stability and interaction in streptokinase. Eur J Biochem 239: 333-339.
Pennica D, Holmes WE, Kohr WJ, Harkins RN, Vehar GA, Ward CA, Bennn WF, Yelverton E, Seeburg PH, Heyneker HL, Goeddel DV, Cohen D. 1982. Clonning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 301: 214-221.
Sumi H, Hamada H, Tsushima H, Mihara H, Muraki H. 1987. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese natto: a typical and popular soybean food in the Japanese diet. Experientia 43: 1110-1111.
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Optimization of the Production of Fibrinolytic Enzyme from Bacillus firmus NA - 1 in Fermented Soybeans
Journal of Food Science and Nutrition
2004 .03
-
된장 유래 혈전분해효소 생산균주의 분리 및 최적 효소생산 조건 탐색
Food Science and Preservation
2005 .12
-
Characterization of a 27 kDa Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1 Isolated from Cheonggukjang
Applied Biological Chemistry
2010 .01
-
Fibrinolytic Enzyme Production by Bacillus subtilis KH - 4 Isolated from Deonjang
Journal of Food Science and Nutrition
2002 .12
-
[P5-40] Fibrinolytic activities of Bacillus Iicheniformis CH3-17 Isolated from Cheonggukjang and Purification of a Fibrinolytic enzyme
한국식품영양과학회 학술대회발표집
2009 .11
-
Characteristic Flavor Compounds of Traditional Korean Chongkukjang
Food Science and Biotechnology
2004 .10
-
한국재래간장 발효균 Bacillus subtilis K7 유래의 혈전용해 Protease의 정제 및 특성
Applied Biological Chemistry
2003 .01
-
Isolation and identification of fibrinolytic enzyme producing strain from Cheonggukjang
한국식품영양과학회 학술대회발표집
2015 .08
-
[S3-3] Expression of Fibrinolytic Enzyme Genes from Bacillus sp. Isolated from Chunggukjang
한국식품영양과학회 학술대회발표집
2007 .10
-
Purification and Characterization of a Fibrinolytic Enzyme from Bacillus pumilus 2.g Isolated from Gembus, an Indonesian Fermented Food
Preventive Nutrition and Food Science
2014 .09
-
Effects of Soaking Solution on Molecular Properties, Solubility, Liquid Holding Capacity, and Viscosity of Soybean Proteins during the Chongkukjang Fermentation
Food Science and Biotechnology
2004 .12
-
Alteromonas sp.가 생산하는 alkaline protease의 특성
Applied Biological Chemistry
1995 .01
-
Isolation and Identification of Fibrinolytic Enzyme Producing Strain from Cheonggukjang
한국식품영양과학회 학술대회발표집
2016 .10
-
Characteristics of Taste Components of Chongkukjang Fermented with Bacillus subtilis
Food Science and Biotechnology
2004 .10
-
Halomonas sp. ES 10이 생산하는 alkaline protease의 특성
Applied Biological Chemistry
1992 .01
-
Fibrinolytic Activity of a Novel Serine Protease from the Hemolymph of a Polychaeta, Periserrula leucophryna
Applied Biological Chemistry
2010 .01
-
[P5-38] Growth and Fibrinolytic Activity of Bacillus licheniformis Strain Carrying a Fibrinolytic Gene, AprE2 from Bacillus subtilis
한국식품영양과학회 학술대회발표집
2007 .10
-
Bacillus subtilis가 생산하는 Alkaline Protease에 관한 연구
Applied Biological Chemistry
1988 .01
-
Anti-fibrinolytic and anti-microbial activities of a serine protease inhibitor from honeybee (Apis cerana) venom
한국양봉학회 학술대회 자료집
2017 .10
이 논문의 저자 정보
이 논문과 함께 이용한 논문
청국장식이가 알코올성지방간 쥐의 지질대사 및 간 기능개선에 미치는 영향
이은희
,
천종희
한국식품영양과학회지
2009
.11
여러 종류 콩의 일반 및 기능성 성분 분석
이솔
,
이윤복
,
김향숙
한국식품영양과학회지
2013
.08
토양 metagenome library로부터 혈전용해효소의 탐색
이선이
,
김보혜
,
강주형
외 5명
생명과학회지
2006
.04
Bacillus subtilis BB-1으로부터 나토키나아제 유전자 크로닝 및 대량발현
이영훈
,
이성호
,
박기훈
외 3명
생명과학회지
2006
.04
향미성 Natto의 Nattokinase, γ - GTP, Protease 활성도와 관능적 평가
김복란
,
이상영
한국식품영양과학회지
1995
.04
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-511-015644618