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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.10
- 수록면
- 548 - 555 (8page)
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초록· 키워드
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Background: Korean Red Ginseng has been used for several decades to treat many diseases, enhancing both immunity and physical strength. Previous studies have documented the therapeutic effects of ginseng, including its anticancer, antiaging, and anti-inflammatory activities. These activities are mediated by ginsenosides present in the ginseng plant. Ginsenoside Rg3, an effective compound from red ginseng, has been shown to have antiplatelet activity in addition to its anticancer and anti-inflammatory activities. Platelets are important for both primary hemostasis and the repair of the vessels after injury; however, they also play a crucial role in the development of acute coronary diseases. We prepared ginsenoside Rg3-enriched red ginseng extract (Rg3-RGE) to examine its role in platelet physiology.
Methods: To examine the effect of Rg3-RGE on platelet activation in vitro, platelet aggregation, granule secretion, intracellular calcium ([Ca<SUP>2+</SUP>]<SUB>i</SUB>) mobilization, flow cytometry, and immunoblot analysis were carried out using rat platelets. To examine the effect of Rg3-RGE on platelet activation in vivo, a collagen plus epinephrine-induced acute pulmonary thromboembolism mouse model was used.
Results: We found that Rg3-RGE significantly inhibited collagen-induced platelet aggregation and [Ca<SUP>2+</SUP>]<SUP>i</SUP> mobilization in a dose-dependent manner in addition to reducing ATP release from collagen-stimulated platelets. Furthermore, using immunoblot analysis, we found that Rg3-RGE markedly suppressed mitogen-activated protein kinase phosphorylation (i.e., extracellular stimuli-responsive kinase, Jun Nterminal kinase, p38) as well as the PI3K (phosphatidylinositol 3 kinase)/Akt pathway. Moreover, Rg3-RGE effectively reduced collagen plus epinephrine-induced mortality in mice.
Conclusion: These data suggest that ginsenoside Rg3-RGE could be potentially be used as an antiplatelet therapeutic agent against platelet-mediated cardiovascular disorders.
Methods: To examine the effect of Rg3-RGE on platelet activation in vitro, platelet aggregation, granule secretion, intracellular calcium ([Ca<SUP>2+</SUP>]<SUB>i</SUB>) mobilization, flow cytometry, and immunoblot analysis were carried out using rat platelets. To examine the effect of Rg3-RGE on platelet activation in vivo, a collagen plus epinephrine-induced acute pulmonary thromboembolism mouse model was used.
Results: We found that Rg3-RGE significantly inhibited collagen-induced platelet aggregation and [Ca<SUP>2+</SUP>]<SUP>i</SUP> mobilization in a dose-dependent manner in addition to reducing ATP release from collagen-stimulated platelets. Furthermore, using immunoblot analysis, we found that Rg3-RGE markedly suppressed mitogen-activated protein kinase phosphorylation (i.e., extracellular stimuli-responsive kinase, Jun Nterminal kinase, p38) as well as the PI3K (phosphatidylinositol 3 kinase)/Akt pathway. Moreover, Rg3-RGE effectively reduced collagen plus epinephrine-induced mortality in mice.
Conclusion: These data suggest that ginsenoside Rg3-RGE could be potentially be used as an antiplatelet therapeutic agent against platelet-mediated cardiovascular disorders.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (36)
참고문헌 신청
[학술지(정기간행물)] - Ghoshal K / 2014 / Overview of platelet physiology : its hemostatic and nonhemostatic role in disease pathogenesis / Sci World J 2014 : 781857 ~
[학술지(정기간행물)] - Davi G / 2007 / Platelet activation and atherothrombosis / N Engl J Med 357 : 2482 ~ 2494
[학술지(정기간행물)] - Grozovsky R / 2015 / Regulating billions of blood platelets : glycans and beyond / Blood 126 : 1877 ~ 1884
[학술지(정기간행물)] - Bye AP / 2016 / Platelet signaling : a complex interplay between inhibitory and activatory networks / J Thromb Haemost 14 : 918 ~ 930
[학술지(정기간행물)] - Barrett NE / 2008 / Future innovations in anti-platelet therapies / Br J Pharmacol 154 : 918 ~ 939
[학술지(정기간행물)] - Davi G / 2007 / Platelet activation and atherothrombosis / N Engl J Med 357 : 2482 ~ 2494
[학술지(정기간행물)] - Grozovsky R / 2015 / Regulating billions of blood platelets : glycans and beyond / Blood 126 : 1877 ~ 1884
[학술지(정기간행물)] - Bye AP / 2016 / Platelet signaling : a complex interplay between inhibitory and activatory networks / J Thromb Haemost 14 : 918 ~ 930
[학술지(정기간행물)] - Barrett NE / 2008 / Future innovations in anti-platelet therapies / Br J Pharmacol 154 : 918 ~ 939
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초음파 처리에 의한 인삼꽃대 엑스의 진세노사이드 성분 변화
생약학회지(Korean Journal of Pharmacognosy)
2016 .03
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UCI(KEPA) : I410-ECN-0101-2018-524-001599322