지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.3
- 수록면
- 236 - 245 (10page)
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초록· 키워드
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Background: Red ginseng (RG) extract, especially ginsenoside Rg1 and Rb1 fractions has been reported to have antithrombotic activities. However, gastric instability and low intestinal permeability are considered to be obstacles to its oral administration. We hypothesized that stability, permeability, and activities of RG might be improved by encapsulation within nanoparticles (NPs) prepared with antithrombotic coating materials.
Methods: RG-loaded chitosan (CS) NPs (PF-NPs) were prepared by complex ionic gelation with the antithrombotic wall materials, polyglutamic acid (PGA), and fucoidan (Fu). The concentrations of PGA (㎎/mL, X₁) and Fu (㎎/mL, X₂) were optimized for the smallest particle size by response surface methodology. Antithrombotic activities of RG and PF-NPs were analyzed using ex vivo and in vivo antiplatelet activities, in vivo carrageenan-induced mouse tail, and arteriovenous shunt rat thrombosis models.
Results: In accordance with a quadratic regression model, the smallest PF-NPs (286 ± 36.6 ㎚) were fabricated at 0.628 ㎎/mL PGA and 0.081 ㎎/mL Fu. The inhibitory activities of RG on ex vivo and in vivo platelet aggregation and thrombosis in in vivo arteriovenous shunt significantly (p ＜ 0.05) increased to approximately 66.82%, 35.42%, and 38.95%, respectively, by encapsulation within PF-NPs. For an in vivo carrageenan-induced mouse tail thrombosis model, though RG had a weaker inhibitory effect, PF-NPs reduced thrombus significantly due to the presence of PGA and Fu.
Conclusion: PF-NPs contributed to improve the activities of RG not only by nanoencapsulation but also by antithrombotic coating materials. Therefore, PG-NPs can be suggested as an efficient delivery system for oral administration of RG.
Methods: RG-loaded chitosan (CS) NPs (PF-NPs) were prepared by complex ionic gelation with the antithrombotic wall materials, polyglutamic acid (PGA), and fucoidan (Fu). The concentrations of PGA (㎎/mL, X₁) and Fu (㎎/mL, X₂) were optimized for the smallest particle size by response surface methodology. Antithrombotic activities of RG and PF-NPs were analyzed using ex vivo and in vivo antiplatelet activities, in vivo carrageenan-induced mouse tail, and arteriovenous shunt rat thrombosis models.
Results: In accordance with a quadratic regression model, the smallest PF-NPs (286 ± 36.6 ㎚) were fabricated at 0.628 ㎎/mL PGA and 0.081 ㎎/mL Fu. The inhibitory activities of RG on ex vivo and in vivo platelet aggregation and thrombosis in in vivo arteriovenous shunt significantly (p ＜ 0.05) increased to approximately 66.82%, 35.42%, and 38.95%, respectively, by encapsulation within PF-NPs. For an in vivo carrageenan-induced mouse tail thrombosis model, though RG had a weaker inhibitory effect, PF-NPs reduced thrombus significantly due to the presence of PGA and Fu.
Conclusion: PF-NPs contributed to improve the activities of RG not only by nanoencapsulation but also by antithrombotic coating materials. Therefore, PG-NPs can be suggested as an efficient delivery system for oral administration of RG.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (53)
참고문헌 신청
Heo JH / 2008 / An open label trial of Korean red ginseng as an adjuvant treatment for cognitive impairment in patients with Alzheimer’s disease / Eur J Neurol 15(8):865~868
Jeon BH / 2000 / Effect of Korea red ginseng on the blood pressure in conscious hypertensive rats / Gen Pharmacol Vasc Syst 35(3):135~141
Vuksan V / 2008 / Korean red ginseng(Panax ginseng)improves glucose and insulin regulation in well-controlled, type 2 diabetes : results of a randomized, double-blind, placebo-controlled study of efficacy and safety / Nutr Metab Cardiovas 18(1):46~56
Furie B / 2005 / Thrombus formation in vivo / J Clin Invest 115(12):3355
Furie B / 2008 / Mechanisms of thrombus formation / N Engl J Med 359(9):938~949
Jeon BH / 2000 / Effect of Korea red ginseng on the blood pressure in conscious hypertensive rats / Gen Pharmacol Vasc Syst 35(3):135~141
Vuksan V / 2008 / Korean red ginseng(Panax ginseng)improves glucose and insulin regulation in well-controlled, type 2 diabetes : results of a randomized, double-blind, placebo-controlled study of efficacy and safety / Nutr Metab Cardiovas 18(1):46~56
Furie B / 2005 / Thrombus formation in vivo / J Clin Invest 115(12):3355
Furie B / 2008 / Mechanisms of thrombus formation / N Engl J Med 359(9):938~949
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