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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 18 - 24 (7page)
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초록· 키워드
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Background: Mesenchymal stromal cells (MSCs) are useful for cell therapy because of their potential for multilineage differentiation. However, MSCs that are expanded in traditional two-di-mensional (2D) culture systems eventually lose their differentiation abilities. Therefore, we investigated whether azacitidine (AZA) supplementation and three-dimensional cul-ture (3D) could improve the differentiation properties of MSCs. Methods: 2D- or 3D-cultured MSCs which were prepared according to the conventional or hang-ing-drop culture method respectively, were treated with or without AZA (1 μM for 72 h), and their osteogenic and adipogenic differentiation potential were determined and compared. Results: AZA treatment did not affect the cell apoptosis or viability in both 2D- and 3D-cultured MSCs. However, compared to conventionally cultured 2D-MSCs, AZA-treated 2D-MSCs showed marginally increased differentiation abilities. In contrast, 3D-MSCs showed sig-nificantly increased osteogenic and adipogenic differentiation ability. When 3D culture was performed in the presence of AZA, the osteogenic differentiation ability was further increased, whereas adipogenic differentiation was not affected. Conclusion: 3D culture efficiently promoted the multilineage differentiation of MSCs, and in combina-tion with AZA, it could help MSCs to acquire greater osteogenic differentiation ability. This optimized culture method can enhance the therapeutic potential of MSCs.
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참고문헌 (25)
참고문헌 신청
Keating A / 2006 / Mesenchymal stromal cells / Curr Opin Hematol 13:419~425
Pittenger MF / 1999 / Multilineage potential of adult human mesenchymal stem cells / Science 284:143~147
Bonab MM / 2006 / Aging of mesenchymal stem cell in vitro / BMC Cell Biol 7:14
Fehrer C / 2005 / Mesenchymal stem cell aging / Exp Gerontol 40:926~930
Papadimitropoulos A / 2014 / Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion / PLoS One 9:e102359
Pittenger MF / 1999 / Multilineage potential of adult human mesenchymal stem cells / Science 284:143~147
Bonab MM / 2006 / Aging of mesenchymal stem cell in vitro / BMC Cell Biol 7:14
Fehrer C / 2005 / Mesenchymal stem cell aging / Exp Gerontol 40:926~930
Papadimitropoulos A / 2014 / Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion / PLoS One 9:e102359
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