지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Bacillus subtilis strain with highly active chitosanase was isolated from the intestine of Sebastiscus marmoratus (scorpion fish). It was named as Bacillus subtilis CH1 by morphological, biochemical and 16S rRNA gene analysis. The optimal conditions for chitosanase production were investigated. The optimum carbon and nitrogen sources for Bacillus subtilis CH1 were 2% starch and 1% yeast extract respectively. Unlike other chitosanases, the expression of this chitosanase was not induced or slightly induced with chitosan. The chitosanase secreted into the medium were concentrated with ammonium sulfate precipitation and purified by gel permeation chromatography. The molecular weight of purified chitosanase was 30 kDa. The optimum pH and temperature of purified chitosanase were 5.5 and 60℃ respectively. The purified chitosanase was continuously thermostable at 40℃ and showed stable activity between pH 6.0 and 8.0. Chitosanase activity of Bacillus subtilis CH1 under optimum condition was 4.1 units/㎖.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgement
References
참고문헌 (28)
참고문헌 신청
[단행본] - Allan, G. G / 1978 / A critical evaluation of potential sources of chitin and chitosan. In Proc. First International Con. on Chitin and Chitosan / MIT Press, Cambridge, MA. : 64 ~ 78
[학술지(정기간행물)] - Boucher, I / 1992 / Purification and characterization of a chitosanase from Streptomyces N174 / Appl. Microbiol. Biotechnol 38 : 188 ~ 193
[단행본] - Davis, B. / 1984 / Chitosanases: occurrence, production and immobilization / Academic Press : 161 ~ 79
[학술지(정기간행물)] - Fenton, D. M / 1981 / Purification and mode of action of a chitosanase from Penicillium islandicum / J. Gen. Microbial 126 : 151 ~ 65
[학술지(정기간행물)] - Hedges, A / 1974 / Extracellular enzyme from Myxobacter Al-l that exhibits both b-l-4 glucanase and chitosanase activities / J. Bacteriol 120 : 844 ~ 53
[학술지(정기간행물)] - Boucher, I / 1992 / Purification and characterization of a chitosanase from Streptomyces N174 / Appl. Microbiol. Biotechnol 38 : 188 ~ 193
[단행본] - Davis, B. / 1984 / Chitosanases: occurrence, production and immobilization / Academic Press : 161 ~ 79
[학술지(정기간행물)] - Fenton, D. M / 1981 / Purification and mode of action of a chitosanase from Penicillium islandicum / J. Gen. Microbial 126 : 151 ~ 65
[학술지(정기간행물)] - Hedges, A / 1974 / Extracellular enzyme from Myxobacter Al-l that exhibits both b-l-4 glucanase and chitosanase activities / J. Bacteriol 120 : 844 ~ 53
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UCI(KEPA) : I410-ECN-0101-2009-529-015613742